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KMID : 1094720110160030611
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Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 3 p.611 ~ p.616
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Improved enzymatic synthesis of N-carbamoyl-D-phenylalanine with in situ product recovery
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Zhou Z.
Yao Z.
Wang H. Q.
Xu H.
Wei P.
Ouyang P. K.
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Abstract
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D-hydantoinase from Burkholderia cepacia was purified and immobilized onto EAH Sepharose 4B using the carbodiimide method. The enzymatic process for the production of N-carbamoyl-D-phenylalanine via the hydantoinase method was conducted using a special packed-bed reactor connected to a DEAE Sepharose FF column for in situ product recovery. This novel apparatus was shown to be effective for pH control, thereby improving the conversion rate. At 40°C, 1.0 g/L substrate concentration, and 10/100 mL adsorbent concentration, the conversion rate of D,L-benzyl hydantoin was 62.7% after the reaction for 14 h, representing an 89.4% increase when compared with that obtained using a packed-bed reactor.
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KEYWORD
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N-D-carbamoyl phenylalanine, bioconversion, immobilized enzyme, packed-bed reactor pH control, in situ product recovery
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